THOC5/FMIP expression is reduced in specific organs. (A, B) 250 μg of poly (I:C) were injected into six-week-old Mx-cre THOC5/FMIP (flox/flox) (n = 8) or control THOC5/FMIP (flox/flox) (n = 6) mice three times at two-to-three-day intervals. THOC5/FMIP protein levels in liver, bone marrow, spleen (A), testicles, lung, kidney, large intestine, and heart (B) were examined by THOC5/FMIP specific immunoblotting. Proteins were extracted from each organ. Total protein amount was standardized by actin specific immunoblotting.
Mancini et al. BMC Biology 2010 8:1 doi:10.1186/1741-7007-8-1