Open Access Research article

Characterization of the histone H2A.Z-1 and H2A.Z-2 isoforms in vertebrates

Deanna Dryhurst1, Toyotaka Ishibashi12, Kristie L Rose34, José M Eirín-López5, Darin McDonald6, Begonia Silva-Moreno1, Nik Veldhoen1, Caren C Helbing1, Michael J Hendzel6, Jeffrey Shabanowitz3, Donald F Hunt37 and Juan Ausió1*

Author Affiliations

1 Department of Biochemistry and Microbiology and The Center for Biomedical Research, University of Victoria, Petch Building, Victoria, BC, V8W 3P6, Canada

2 California Institute for Quantitative Biosciences, University of California, Berkeley, 642 Stanley Hall, Berkeley, CA, 94720-3220, USA

3 Department of Chemistry, University of Virginia, Charlottesville, VA, 22904, USA

4 The Scripps Research Institute, 130 Scripps Way #1B2, Jupiter, FL, 33458, USA

5 Departamento de Biología Celular y Molecular, Universidade da Coruña, E15071 A Coruña, Spain

6 Department of Oncology, University of Alberta and Cross Cancer Institute, Edmonton, Alberta, T6G 1Z2, Canada

7 Department of Pathology, University of Virginia, Charlottesville, VA, 22904, USA

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BMC Biology 2009, 7:86  doi:10.1186/1741-7007-7-86

Published: 14 December 2009

Additional files

Additional file 1:

Nucleotide alignment of H2A.Z-1 and H2A.Z-2 proximal promoter sequences from representative mammals (human, rhesus monkey and mouse). Regulatory sequences in H2A.Z-1 are indicated by solid boxes in yellow (TATA box), green (GC box) and blue (CAAT box). Regulatory elements in H2A.Z-2 are underlined in red (SP1 TP1 binding sites) and purple (c-Myc binding sites). Numbering above the alignment represents the number of nucleotides from the origin of transcription.

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Additional file 2:

Nucleotide alignment of H2A.Z-1 and H2A.Z-2 upstream promoter region sequences from representative mammals (human, rhesus monkey and mouse). Regulatory elements in H2A.Z-1 are indicated by solid boxes in red (GC boxes), blue (CAAT boxes), purple (c-Myc binding sites), and by an open box (BGP1 RS1 binding site). Regulatory elements in H2A.Z-2 are underlined in red (SP1 TR1 binding sites), purple (c-Myc binding sites), blue (CAAT boxes), green (LyF Ikaros binding site), brown (N-Myc binding sites) and by open boxes (BGP1 RS1 binding sites). Numbering above the alignment represents the number of nucleotides from the origin of transcription.

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Additional file 3:

GenBank Accession numbers for the histone variants H2A.Z-1 and H2A.Z-2 used in the present work. The ANNOTATION field denotes: gene sequences newly isolated from draft genomes (in silico), gene sequences predicted as either H2A.Z-1 or H2A.Z-2 from databases and draft/complete genomes data (Pred), sequences defined either as H2A.Z-1 or H2A.Z-2 by the present analyses (a), sequences defined as H2A by the present analyses (b) and sequences whose annotation either as H2A.Z-1 or H2A.Z-2 has been corrected by the present work (c).

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