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Open Access Methodology article

Fluorescence resonance energy transfer (FRET)-based subcellular visualization of pathogen-induced host receptor signaling

Alexander Buntru12, Timo Zimmermann3 and Christof R Hauck12*

Author Affiliations

1 Lehrstuhl für Zellbiologie, Universität Konstanz, Konstanz, Germany

2 Konstanz Research School Chemical Biology, Universität Konstanz, Konstanz, Germany

3 Advanced Light Microscopy Unit, CRG-Centre de Regulació Genòmica, Barcelona, Spain

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BMC Biology 2009, 7:81  doi:10.1186/1741-7007-7-81

Published: 25 November 2009

Additional files

Additional file 1:

Movie 1. Hck-Src homology 2 (SH2) domain is transiently recruited to wild type carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3 WT) at sites of bacterial infection. 293T cells were transfected to express far-red fluorescent protein (mKate)-Hck-SH2 and CEACAM3 WT-enhanced green fluorescent protein (EGFP). After addition of AF647-labeled OpaCEA-expressing Neisseria gonorrhoeae the infection process was monitored for 2 h. Scale bar: 10 μm.

Format: MOV Size: 8.3MB Download file

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Open Data

Additional file 2:

Movie 2. Hck-Src homology 2 (SH2) domain is not recruited to carcinoembryonic antigen-related cell adhesion molecule 3 without the cytoplasmic domain (CEACAM3 ΔCT), even though bacteria still bind to the receptor. 293T cells were transfected to express far-red fluorescent protein (mKate)-Hck-SH2 and CEACAM3 ΔCT-enhanced green fluorescent protein (EGFP). After addition of AF647-labeled OpaCEA-expressing Neisseria gonorrhoeae the infection process was monitored for 2 h. Scale bar: 10 μm.

Format: MOV Size: 16.4MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data