BMC Biology

official impact factor 5.20

Open Access Highly Access Research article

Environmentally-acquired bacteria influence microbial diversity and natural innate immune responses at gut surfaces

Imke E Mulder1, Bettina Schmidt1, Christopher R Stokes2, Marie Lewis2, Mick Bailey2, Rustam I Aminov1, James I Prosser3, Bhupinder P Gill4, John R Pluske5, Claus-Dieter Mayer6, Corran C Musk1 and Denise Kelly1*

Author Affiliations

1 Gut Immunology Group, University of Aberdeen, Rowett Institute of Nutrition and Health, Greenburn Road, Aberdeen AB21 9SB, UK

2 Veterinary Pathology, Infection & Immunity, Langford House, Langford, Bristol, BS40 5DU, UK

3 Institute of Biological and Environmental Sciences, University of Aberdeen, St Machar Drive, Aberdeen AB24 3UU, UK

4 Agricultural and Horticultural Development Board, Winterhill House, Snowdon Drive, Milton Keynes MK6 1AX, UK

5 School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia

6 Biomathematics & Statistics Scotland, University of Aberdeen, Rowett Institute of Nutrition and Health, Greenburn Road, Aberdeen AB21 9SB, UK

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BMC Biology 2009, 7:79 doi:10.1186/1741-7007-7-79

Published: 20 November 2009

Additional files

Additional file 1:

Enumeration of Lactobacillus species.

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Additional file 2:

Transcripts differentially expressed between treatments at all three time-points. Differentially expressed genes at each time point are shown for the comparison IR versus OUT, and IN versus OUT (P < 0.01, -2 ≤ fold change ≥ 2, N = 6).

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Additional file 3:

Real-time PCR primer sequences. Porcine gene-specific primers were designed for the sequence of interest using Primer Express Software v3.0 (Applied Biosystems).

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Open Data