Table 1 |
||
|
Comparison between H14 and assay media. |
||
|
Composition |
H14 medium |
Assay medium |
|
|
||
|
Type of medium |
DMEM/F12 |
DMEM/F12 |
|
Prolactin |
5 μg/ml |
- |
|
Insulin |
250 ng/ml |
10 μg/ml |
|
Hydrocortisone |
1.4 μg/ml |
0.5 μg/ml |
|
17-Beta-estradiol |
10-10 M |
- |
|
Sodium Selenite |
2.6 ng/ml |
- |
|
Transferrin |
10 μg/ml |
- |
|
EGF |
10 ng/ml |
5 ng/ml |
|
Horse serum |
- |
2% |
|
Cholera toxin |
- |
100 ng/ml |
|
Antibiotics |
- |
50 U/ml penicillin 50 μg/ml streptomycin |
|
% of Matrigel™ drip for 3D culture |
5% |
2% |
|
|
||
|
2D culture process for passages |
culture for 8 to 12 days |
culture for 3 to 4 days Medium different from 3D culture medium (5% horse serum, 20 ng/ml EGF) |
|
|
||
|
Plachot et al. BMC Biology 2009 7:77 doi:10.1186/1741-7007-7-77 |
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