Table 1

Comparison between H14 and assay media.

Composition

H14 medium

Assay medium


Type of medium

DMEM/F12

DMEM/F12

Prolactin

5 μg/ml

-

Insulin

250 ng/ml

10 μg/ml

Hydrocortisone

1.4 μg/ml

0.5 μg/ml

17-Beta-estradiol

10-10 M

-

Sodium Selenite

2.6 ng/ml

-

Transferrin

10 μg/ml

-

EGF

10 ng/ml

5 ng/ml

Horse serum

-

2%

Cholera toxin

-

100 ng/ml

Antibiotics

-

50 U/ml penicillin

50 μg/ml streptomycin

% of Matrigel™ drip for 3D culture

5%

2%


2D culture process for passages

culture for 8 to 12 days

culture for 3 to 4 days

Medium different from 3D culture medium (5% horse serum, 20 ng/ml EGF)


Plachot et al. BMC Biology 2009 7:77   doi:10.1186/1741-7007-7-77

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