HTP culture produces a basoapical polarity axis similar to that shown with classical 3D-Matrigel™ cultures. A. Organization of the basoapical polarity axis in 2D and HTP cultures of S1 cells as shown by immmunofluorescence staining of basal (collagen IV [green], α6-integrin [green]), lateral [beta-catenin [red] and desmoplakin 1,2 [red]), lateroapical [ZO-1 [green]), and apical (mucin-1 [green]) polarity markers. Nuclei are counterstained with DAPI (blue). Size bar, 5 μm. B. drawings of the different cell culture systems used in this study (2D monolayer; Matrigel™-embedded: cells totally embedded in the Matrigel™ gel; 3D-Matrigel™ drip: 5% Matrigel™ drip on Matrigel™ gel-coated surface; HTP: 5% Matrigel™ drip on glass surface). The gel is represented in red; soluble Matrigel™ dripped in the culture medium is represented by curvy lines.
Plachot et al. BMC Biology 2009 7:77 doi:10.1186/1741-7007-7-77