Apically localized tight junctions in mammary acini. A. Apical concentration of tight junction proteins PALS1 and PAR3 as shown by immunohistochemistry [reddish-brown; arrows] in acini from normal looking mammary tissue (from biopsy sections - left panel), and acini formed by S1 cells in 3D-Matrigel™ drip culture (center panel) as shown by immunofluorescence [green]. Diffuse distribution of PALS1 and PAR3 in multicellular structures formed by MCF10A cells in 3D culture (right panel). Nuclei are counterstained with 4', 6-diamidino-2-phenylindole (DAPI) (blue) in cell cultures and hematoxylin (blue) in IHC. Size bar, 5 μm. B. Electron micrograph of a section of an acinus formed by S1 cells in 3D culture. Insets show specific adhesion complexes. Size bar, 1 μm; A = apical; B = basal; L = lumen.
Plachot et al. BMC Biology 2009 7:77 doi:10.1186/1741-7007-7-77