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Open Access Research article

Predominant membrane localization is an essential feature of the bacterial signal recognition particle receptor

Miryana Mircheva1, Diana Boy1, Benjamin Weiche1, Friederike Hucke2, Peter Graumann2 and Hans-Georg Koch1*

Author Affiliations

1 Institut für Biochemie und Molekularbiologie, ZBMZ, Universität Freiburg, Stefan-Meier-Str. 17, 79104 Freiburg, Germany

2 Mikrobiologie, Fakultät für Biologie, Universität Freiburg, Schänzlestraße 1, 79104 Freiburg, Germany

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BMC Biology 2009, 7:76  doi:10.1186/1741-7007-7-76

Published: 13 November 2009

Additional files

Additional file 1:

Movie S1 - Membrane localization of green fluorescent protein (GFP)-tagged wild type FtsY. GFP-tagged wild type FtsY was expressed from a plasmid in DH5α and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. Z stacks were acquired with 0.1 μm spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player.

Format: MOV Size: 419KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional file 2:

Movie S2 - Membrane localization of green fluorescent protein (GFP)-tagged FtsY(B3) mutant derivative. The GFP-tagged FtsY(B3) mutant derivative was expressed from a plasmid in DH5α and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. The FtsY(B3) derivative exhibits reduced activity due to a triple-point mutation within the first lipid-binding domain of FtsY. Z stacks were acquired with 0.1 μm spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player.

Format: MOV Size: 518KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data

Additional file 3:

Movie S3 - Membrane localization of green fluorescent protein (GFP)-tagged FtsY(NG-1) mutant derivative. The GFP-tagged FtsY(NG+1) derivative was expressed from a plasmid in DH5α and localization was monitored on a motorised Zeiss Axioobserver microscope using a Cascade EM-CCD camera. The FtsY(NG+1) is an N-terminally truncated FtsY-derivative, which contains only one out of two lipid binding domains and displays reduced activity. Z stacks were acquired with 0.1 μm spacing and were three dimensionally de-convoluted using Autodeblur software. Movies were created in Metamorph and can be displayed with Quicktime movie player.

Format: MOV Size: 821KB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Open Data