Additional file 4.
JNKDN represses scrib1 + RasACT tumour overgrowth and invasion. Pairs of larval eye/antennal imaginal discs attached to brain lobes (bl) containing eyFLP-induced MARCM clones (green) at day 5 (A, B, F, G), day 7 (C, D) and day 8 (E). Grey scale is Elav (A, F). Red is phalloidin to mark F-actin. (A-B) UAS-dRas1V12; FRT82B. RasACT-expressing clones do not massively overgrow and mutant cells are not observed between the brain lobes. Note the F-actin rich cables (arrows) extending from between the eye/antennal disc to the region between the brain lobes. (C-D) UAS-dRas1V12; FRT82B scrib1. scrib1 + RasACT tumours massively overgrow by day 7 and tumour cells appear to migrate between the brain lobes (arrow in C) along F-actin rich cables (arrow in D). (E) UAS-dRas1V12; FRT82B crb11A22 scrib1. Loss of crb does not abrogate scrib1 + RasACT tumour overgrowth. (F, G) UAS-dRas1V12; FRT82B scrib1 UAS-bskDN. Expression of BskDN in scrib1 + RasACT tumours prevents tumour overgrowth throughout an extended larval stage of development and blocks invasion of tumour cells between the brain lobes.
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Leong et al. BMC Biology 2009 7:62 doi:10.1186/1741-7007-7-62