Table 1 |
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|
Characteristic kinetic constants for the hydrolysis of ATCh and BTCh by DmAChE, HuAChE and HuBChE |
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|
DmAChE (ATCh) |
HuAChE (ATCh) |
HuBChE (BTCh) |
|
|
|
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|
Substrate |
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|
k3 [s-1] |
395.4 |
13471 ± 2171 |
1082 ± 44 |
|
Kip [mM] |
0.17 |
5.43 ± 1.0 |
11.3 ± 0.5 |
|
KL |
4.17 |
0.0181 ± 0.0054 |
0.0019 ± 0.0008 |
|
KLL |
179 |
9.23 ± 6.58 |
12.3 ± 5.4 |
|
k2 [s-1] |
53465 |
14375 ± 2182 |
420 ± 20 |
|
A |
3.44 |
1.64 ± 0.36 |
1.32 ± 0.07 |
|
B |
0.049 |
0.11 ± 0.03 |
39.6 ± 13.5 |
|
Ks = Kp*KL [mM] |
0.73 |
0.098 |
0.021 |
|
Kss = Kp*KLL [mM] |
31.1 |
50.1 |
139 |
|
|
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|
DEET |
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|
Kip [mM] |
1.02 ± 0.03 |
8.39 ± 6.97 |
0.37 ± 0.05 |
|
KiL |
0.083 ± 0.078 |
1.54 ± 0.45 |
|
|
KiLL |
0.56 ± -0.41 |
29 ± 5.4 |
|
|
c |
1.49 ± 0.99 |
1.32 |
|
|
d |
0.19 ± 0.18 |
0.52 ± 0.09 |
|
|
Kis = Kip*KiL [mM] |
0.69 |
0.57 |
|
|
Kiss = Kip*KiLL [mM] |
4.67 |
1.08 |
|
|
|
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ATCh = acetylthiocholine; BTCh = butyrylthiocholine; DmAChE = Acetylcholinesterase from D. melanogaster; HuAChE = Acetylcholinesterase from human; HuBChE = Butyrylcholinesterase from human. |
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|
Corbel et al. BMC Biology 2009 7:47 doi:10.1186/1741-7007-7-47 |
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