Table 1

Characteristic kinetic constants for the hydrolysis of ATCh and BTCh by DmAChE, HuAChE and HuBChE

DmAChE (ATCh)

HuAChE (ATCh)

HuBChE (BTCh)


Substrate

k3 [s-1]

395.4

13471 ± 2171

1082 ± 44

Kip [mM]

0.17

5.43 ± 1.0

11.3 ± 0.5

KL

4.17

0.0181 ± 0.0054

0.0019 ± 0.0008

KLL

179

9.23 ± 6.58

12.3 ± 5.4

k2 [s-1]

53465

14375 ± 2182

420 ± 20

A

3.44

1.64 ± 0.36

1.32 ± 0.07

B

0.049

0.11 ± 0.03

39.6 ± 13.5

Ks = Kp*KL [mM]

0.73

0.098

0.021

Kss = Kp*KLL [mM]

31.1

50.1

139


DEET

Kip [mM]

1.02 ± 0.03

8.39 ± 6.97

0.37 ± 0.05

KiL

0.083 ± 0.078

1.54 ± 0.45

KiLL

0.56 ± -0.41

29 ± 5.4

c

1.49 ± 0.99

1.32

d

0.19 ± 0.18

0.52 ± 0.09

Kis = Kip*KiL [mM]

0.69

0.57

Kiss = Kip*KiLL [mM]

4.67

1.08


ATCh = acetylthiocholine; BTCh = butyrylthiocholine; DmAChE = Acetylcholinesterase from D. melanogaster; HuAChE = Acetylcholinesterase from human; HuBChE = Butyrylcholinesterase from human.

Corbel et al. BMC Biology 2009 7:47   doi:10.1186/1741-7007-7-47

Open Data