TBP2 is associated with active promoters in oocytes. (A) Chromatin immunoprecipitation (ChIP) assay on germinal vesical (GV)-injected promoters and endogenous 5S rRNA promoters using TBP2 and pol-II antibodies. Enrichment is the signal relative to background which in this case is the ChIP recovery from a non-promoter region (intron construct). (B) ChIP assay on GV-injected pol-II promoters and endogenous 5S rRNA promoter from oocytes injected with HA-TBP2 mRNA. Enrichment is the signal relative to background which in this case is the ChIP signal from oocytes not expressing HA-TBP2 but injected with the same promoter construct. Error bars in (A) and (B) represent the standard error of the mean from three independent experiments.
Akhtar and Veenstra BMC Biology 2009 7:45 doi:10.1186/1741-7007-7-45