Reversibility, efficiency, and competition tests. (A) Reversible lethality: three-day-old flies from LLs #66 and #67 were reared on Tc-containing food (+Tc; 10 μg/ml) for two days, transferred to Tc-free medium (-Tc) for five days and transferred back to Tc-containing food for three days. Progeny of 24 h egg lay intervals were monitored (embryos from Tc-containing or Tc-free adult medium were reared on 1 μg/ml Tc-containing or Tc-free larval food, respectively). The ratio of adults to laid eggs is shown. For comparison, the ratio of eclosed adults to laid eggs in wildtype (WT) was in a range of 54%–74% under our rearing conditions (not shown). Two repetitions of the time series were performed. The SD of these two repetitions is indicated. Differences between repetitions are non-significant (ns), as shown by chi-test (Additional file 5). (B) Efficiency test: Shown are the combined data of four repetitions (see also Additional file 3). Total hatched L1 larvae 48 h after egg collection, total pupae, and total adults were counted and are shown in relation to the total number of eggs (total egg number: n (#29) = 1499; n (#72) = 4330; n (#66) = 2278; n (#67) = 2058; n (#68) = 1914; n (WT) = 2411). Due to difficulties in the larval count, the number of surviving larvae might be an under-representation. The SD of four repetitions is indicated. Differences between repetitions are ns, as shown by t-tests (Additional file 5). (C) Competition for virgin WT females: Numbers are normalized to the positive control (1:1:0). The expected larval hatch rate is indicated in brackets. The SD of two repetitions (each independent repetition consisting of the six egg collections) is indicated. Differences between repetitions are ns, as shown by t-tests (Additional file 5).
Schetelig et al. BMC Biology 2009 7:4 doi:10.1186/1741-7007-7-4