tTA and hidAla5 expression depends on different P/Es. Expression of tTA and hidAla5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly lines carrying both driver and effector constructs in homozygous condition. The developmental stages of embryogenesis are indicated to the right of the respective panels. The lines carry driver constructs with different P/E (P) driving the tTA. The depicted lines are representative for independent lines (three for sl1, two for sl2, three for 99, and one for CG2186) carrying the respective driver construct. All presented lines derive from the effector line TREhs43-hidAla5_F1m2 and were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.
Schetelig et al. BMC Biology 2009 7:4 doi:10.1186/1741-7007-7-4