Analysis of Aplysia Gαq, Gαi, and Gαo proteins in rhinophore. (a) Schematic diagrams of Gαq (GenBank: DQ397515), Gαi (GenBank: DQ656111) and Gαo (GenBank: DQ656112) showing the location of N-terminal cysteines that may be sites for palmitoylation (wavy lines), a putative cholera toxin ADP-ribosylation site (●), and the binding site of Gα-specific antibodies used for immunolocalization analyses. (b to d) Immunofluorescent (green) localization of Gαq (b), Gαi (c) and Gαo (d) in the rhinophore sensory epithelium (SE), including presumptive sensory neuron cell fibers (arrows) and outer epithelia (arrowheads). Sections were counterstained with propidium iodide (red). Scale bars = 100 μm. Higher magnifications are shown in insets.
Cummins et al. BMC Biology 2009 7:28 doi:10.1186/1741-7007-7-28