Table 1

Effect of surface sterilization of eggs on symbiont acquisition in Elasmostethus humeralis.

Symbiont detection (no. positive/total)


Egg mass number

Control

Surface sterilization a,b


1

6/6

0/6

2

8/8

0/12

3

15/16

0/18

4

13/13

0/14

5

7/8

0/9

6

6/7

0/7

7

7/7

0/6

8

20/20

0/18


Total

82/85

0/90


aEggs were treated with 70% ethanol for 10 min and 4% formaldehyde containing 0.1% Triton X-100 for 30 min, and rinsed two times with 70% ethanol.

bAll samples showed no amplification after 35 cycles of PCR.

Kikuchi et al. BMC Biology 2009 7:2   doi:10.1186/1741-7007-7-2

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