Figure 6.

Negative relationship of toll-like receptor (TLR)-mediated responses with louse (Polyplax serrata) infection grade. A-D) Scatter of TLR-response variables at increasing louse infection grades. Box plots show the individual scatter of data, the median (line), the interquartile range (box) and the range between the highest and lowest values (whiskers) excluding outliers (values differing by more than 1.5 times the interquartile range interval from the nearest limit of the interquartile range). A) Overall TLR-mediated responsiveness represented by first principal component (PC1^I) scores from a principal components analysis (PCA) of the six log-transformed TLR variables. B) Log-transformed TLR2 response against HKLM. C) Log-transformed TLR2 response against zymosan. D) Log-transformed TLR9 response against ODN2006. E-F) Confounder-adjusted general linear mixed model predictions for the effect of louse grade on untransformed TLR-mediated tumour necrosis factor alpha (TNF-α) responses (pg/ml) with other model terms averaged. One standard error is shown above and below each estimate. E) TLR2 and TLR9-mediated responses. F) Total TLR-mediated response (summed across all six TLR variables), TIR.