LALBA reporter gene expression. (A) Cape fur seal (CFS), Cape fur seal TATA box mutation (CFSmut) and harbour seal (HS) reporter gene LALBA expression (718 bp promoter) in the presence of an introduced STAT5 site in BME-UV1 cells in the presence of insulin (I) or insulin/prolactin/dexamethazone (IPDEX) induction. (B) Reporter gene LALBA expression (398 bp promoter) in the presence of a pGL3 enhancer site in BME-UV1 and (C) HEK293T cells. Statistical analysis showed HS reporter gene expression in BME-UV1 cells is significantly higher than CFS in a STAT5 background (P = 0.038) and also in an Enhancer background in BME-UV1 cells (P = 0.000) and HEK239T cells (P = 0.010). Experiments were performed in duplicate and repeated at least twice. Standard errors are shown.
Sharp et al. BMC Biology 2008 6:48 doi:10.1186/1741-7007-6-48