Over-expression of OFUT1 inhibits Notch activity non-enzymatically and non-autonomously. Adult nota from (A) ap-Gal4 control, (B) ap-Gal4; UAS-Ofut1 [8.2]and (C) ap-Gal4; UAS-Ofut1R245A[28.3]. Note the increased density of bristles (arrows) when OFUT1 is over-expressed. All of the crosses are performed at 25°C. The average number of microchaetes present in the acrostichal region of the notum are as follows: (A) 97 ± 10, n = 3; (B) 147 ± 25, n = 3; (C) 140 ± 15, n = 4. (D) WG expression (red) in a wild-type wing disk. (E) WG expression (red) in a ptc-Gal4 UAS-GFP; UAS-Ofut1 [11.1] wing disk. WG expression along the D-V boundary is decreased both inside (arrow) and outside (arrowhead) of the ptc stripe (green).
Okajima et al. BMC Biology 2008 6:1 doi:10.1186/1741-7007-6-1