Table 2 |
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|
Primers used for the quantitative real-time PCR. |
|||
|
UniGene |
Gene name |
FP/RP |
Sequence (5'-3') |
|
|
|||
|
Rn.1079 |
Arbp |
FP |
AGAGTCGGAGGAATCCGATGA |
|
RP |
AAGCAGGCTGACTTGGTGTGA |
||
|
Rn.38575 |
Ncf1 |
FP |
CGAGAGTGGCTGGTGGTT |
|
RP |
CATACGGTTCACCTGCGTAGT |
||
|
Rn.5078 |
Ass |
FP |
TGTTCGCCACTGCATCGA |
|
RP |
TGGCCCTTGAAGACAGATACCT |
||
|
Rn.7391 |
Cxcl9 |
FP |
ACGGAGATCAAACCTGCCTAGAC |
|
RP |
CATCAGCTTCTTCACCCTTGCT |
||
|
Rn.33193 |
Mmp12 |
FP |
AGGTACGATGTGAGGCAGGAA |
|
RP |
TCCTGGGAAGTGTGTAGAAATCAG |
||
|
Rn.14882 |
Best5 |
FP |
TCATTCTGCGTTGGAGTTTGG |
|
RP |
CTGAATTATTGATGGGAGGAGTCA |
||
|
Rn.101159 |
IRF7 |
FP |
CAGAACCCCAACTTCCAATAGAGT |
|
RP |
TAGAGATGACATATAGCCAAGGAATAAGC |
||
|
Rn.35775 |
Ifit3 |
FP |
TGGGAGGCAACACGATTTG |
|
RP |
TGACACACTTCCGGTTGTTATCAG |
||
|
Rn.10383 |
Oas1 |
FP |
GGATCACAGTCCATGCTTCTGA |
|
RP |
CTGTGATTGGACAGTATTCAAACATG |
||
|
Rn.10374 |
Mx2 |
FP |
GTGACACCAGTGAGAAGAGGAGATT |
|
RP |
GCTAGCCTGCGCTGAGCTT |
||
|
Rn.6703 |
S100a9 |
FP |
TGGACACAAACCAGGACAATCA |
|
RP |
CTCATGACAGGCAAAGATCAACTT |
||
|
Rn.143194 |
Ifnα 1 |
FP |
GCAACCCTCCTAGACTCATTCTG |
|
RP |
ACCCCTACCTGCTGCATCAG |
||
|
Rn.138105 |
Ifnβ 1 |
FP |
CTTGGGTGACATCCACGACTAC |
|
RP |
GGCATAGCTGTTGTACTTCTTGTCTT |
||
|
Rn.10795 |
Ifnγ |
FP |
AATCGCACCTGATCACTAACTTCTT |
|
RP |
TGGGTTGTTCACCTCGAACTT |
||
|
|
|||
|
The final concentration of each primer in the sample was 400 nM, except for Ifnβ 1, for which 800 nM was used. FP, forward primer; RP, reverse primer. |
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|
Olofsson et al. BMC Biology 2007 5:19 doi:10.1186/1741-7007-5-19 |
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