Table 1

Tetracycline-repressible lethality in LA513A.

Cross

Parents

Progeny


Male

Female

Tc

Genotype

Egg

L1

L2

L3

L4

Pupae

Adults

%


a

LA513A/+

+/+

Yes

LA513A/+

1000

489

468

446

442

437

434

89

Wild type

444

431

403

400

396

392

88

b

+/+

LA513A/+

Yes

LA513A/+

1000

442

420

404

399

393

383

87

Wild type

466

444

428

417

412

404

87

c

LA513A/+

+/+

No

LA513A/+

540

274

265

235

208

155

7

2.6

Wild type

233

225

214

212

209

206

88

d

+/+

LA513A/+

No

LA513A/+

497

216

205

181

168

131

9

4.2

Wild type

241

225

216

214

211

207

86


Adults heterozygous for LA513A were allowed to mate with wild type. a and c: transgenic males crossed to wild type females; b, d: the reciprocal cross using transgenic females. Eggs were collected and the resulting larvae reared in media supplemented with tetracycline (Tc) to 30 μg/ml (crosses a and b) or in normal, tetracycline-free media (crosses c and d). Data are the sum of at least 5 experiments. The ratio of transgenic to non-transgenic first instar larvae (L1) was approximately 1:1 (1421:1384), indicating that there was no significant differential embryonic mortality between transgenic and wild type (binomial exact test,p = 0.248). The numbers of transgenic and non-transgenic first, second, third and fourth instar larvae (L1–L4), pupae and adults resulting from these eggs is shown. In the absence of tetracycline, the transgenics showed very high (96–97%) mortality between first instar larvae and adult stages (highlighted cells); this was completely suppressed by tetracycline. Mortality of transgenics in the absence of tetracycline is strongly stage-specific, being primarily around the pupal stage (L4-pupae and pupae-adult; most affected individuals started to pupate but failed to develop beyond the earliest stages of pupal development).

Phuc et al. BMC Biology 2007 5:11   doi:10.1186/1741-7007-5-11

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