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Open Access Highly Accessed Research article

A rapidly evolving secretome builds and patterns a sea shell

Daniel J Jackson12, Carmel McDougall14, Kathryn Green1, Fiona Simpson3, Gert Wörheide2 and Bernard M Degnan1*

Author Affiliations

1 School of Integrative Biology, University of Queensland, Brisbane Qld 4072, Australia

2 Department of Geobiology, Geoscience Centre, University of Göttingen, Goldschmidtstr.3, 37077 Göttingen, Germany

3 Institute of Molecular Biosciences, University of Queensland, Brisbane Qld 4072, Australia

4 Department of Zoology, University of Oxford, Tinbergen Bldg., South Parks Road, Oxford OX1 3PS, UK

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BMC Biology 2006, 4:40  doi:10.1186/1741-7007-4-40

Published: 22 November 2006

Additional files

Additional File 1:

Table 1: Complete list of H. asinina mantle ESTs and associated TBLASTX and BLASTN results.

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Additional File 2:

Table 2: Genes reported to be involved in molluskan biomineralization that were searched against the Lottia scutum genome.

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Additional File 3:

Table 3: H. asinina mantle ESTs and biomineralization genes that share significant similarity with a Lottia scutum genomic trace.

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Additional File 4:

Fig. 5: Phylogenetic analysis of Has-sometsuke (HasSom). (a) An unrooted parsimony tree (1000 bootstrap replicates) and (b) an unrooted Bayesian phylogram (200,000 generations, 1,000 burn in trees) illustrate the divergent nature of both Haliotis asinina and Strongylocentrotus purpuratus ependymin-like sequences from previously reported ependymin sequences. In agreement with a previous phylogenetic analysis [56], the echinoderms Lytechinus variegatus and Holothuria glaberima form a clade in association with the mammalian and Xenopus sequences. The amphioxus and Strongylocentrotus purpuratus sequences were not included in this previous analysis. (c) The protein alignment used to generate the trees shown in (a) and (b). Accession numbers are indicated. Alignments were created with both ClustalW and Dialign, compared and manually optimized. Arrows indicate conserved cysteine residues that are characteristic of the ependymin proteins [54]. Red arrow indicates the presence of a cysteine residue only in the H. asinina, S. purpuratus and amphioxus sequences. Asterisk indicates the loss of a cysteine residue in S. purpuratus. Figures following alignments are percentage identities and percentage positives respectively and were generated by significant pairwise bl2seq alignments between HasSom and each individual sequence.

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