Table 2

The unclassified sequence from the Sargasso Sea is unlikely to represent a fragment of a viral genome. TBLASTN searches were conducted at NCBI using as a query the 59 residues from the Sargasso Sea sequence (Accession AACY01369547) that formed the putative C-extein. These 59 residues are encoded on the complementary strand, from base 556 to base 732. Each search was restricted to one of the six groups outlined below.

Accession

Sequences producing significant alignments

E value


Fungi

gb DQ302778.1

Entomophthora muscae AFTOL-ID28, RPB2

4 × 10-9

gb DQ521419.1

Auricularia auricula-judae AFTOL-ID1681

7 × 10-9

gb DQ234553.1

Hydnum albomagnum AFTOL-ID 471, RPB2

1 × 10-8

gb AY485624.1

Hydnum repandum RPB2

1 × 10-8

gb DQ302787.1

Umbelopsis ramanniana AFTOL-ID 144, RPB2

2 × 10-8

Metazoa

gb XM_793194.1

Strongylocentrotus purpuratus LOC593725

5 × 10-7

dbj AK114672.1

Ciona intestinalis cDNA, clone:cieg010h22

1 × 10-6

gb AC007441.9

Drosophila melanogaster clone BACR10E03

2 × 10-6

gb BT028050.1

Gasterosteus aculeatus clone CNB114-G10

2 × 10-6

gb U10333.1

Caenorhabditis elegans RNA polymerase II

2 × 10-6

Plantae

emb AJ565937.1

MGU565937 Mimulus guttatus partial RPB2

9 × 10-7

gb DQ029103.1

Spirogyra sp. UWCC FW670 RPB2

1 × 10-6

gb AF020844.1

Marchantia polymorpha RPB140 (RPB2)

1 × 10-6

gb AY596718.1

Tetralocularia pennelii (RPB2)

1 × 10-6

gb AY563264.1

Selaginella densa RNA polymerase II (RPB2)

1 × 10-6

emb AJ566358.1

GSP566358 Gardenia sp. Oxelman 2319 (RPB2)

1 × 10-6

Archaea

gb AE010299.1

Methanosarcina acetivorans str. C2A (rpoB)

2 × 10-6

gb AE008384.1

Methanosarcina mazei strain Goe1 (rpoB)

2 × 10-6

gb CP000099.1

Methanosarcina barkeri str. fusaro

3 × 10-6

gb AE000782.1

Archaeoglobus fulgidus DSM 4304 (rpoB1)

4 × 10-6

emb BX957222.1

Methanococcus maripaludis S2

5 × 10-6

emb X14818.1

Sulfolobus acidocaldarius rpoB

9 × 10-6

Viruses

gb AF389451.1

Tiger frog virus, complete genome

2 × 10-4

gb AY548484.1

Frog virus 3, complete genome

4 × 10-4

gb AY666015.1

Grouper iridovirus, complete genome

0.001

gb AY150217.1

Ambystoma tigrinum stebbensi virus

0.001

gb AF397202.1

Regina ranavirus clone PstI-3.8

0.001

gb AY653733.1

Acanthamoeba polyphaga Mimivirus

0.001

emb AJ890364.1

Emiliania huxleyi virus 86 isolate Ehv86

0.003

Eubacteria

No significant similarity found.


Goodwin et al. BMC Biology 2006 4:38   doi:10.1186/1741-7007-4-38

Open Data