Sstr2 and CCR5 transgenic animals mutants are desensitised by pre-exposure to agonists. (A) Pre-exposure of Sstr2 transgenic animals to 1 μM and 10 μM SST-28 prior to the assay strongly reduced or even abolished avoidance behaviour. (B)Pre-exposure of CCR5 transgenic animals to 1 μM and 10 μM MIP-1α fully abolished avoidance of 1 μM, 10 μM and 25 μM MIP-1α. In both cases, pre-exposure to the agonist did not affect the responses towards other repellents. Animals were washed in buffer as control. For each panel, each data point represents an average of at least five independent assays for wild type and each respective strain. Error bars denote the standard error of the mean. Asterisks indicate a statistically significant difference between transgenic animals pre-exposed to agonist or only to chemotaxis (CTX) buffer (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005).
Teng et al. BMC Biology 2006 4:22 doi:10.1186/1741-7007-4-22