Heterologously expressed GPCRs are integrated into an endogenous signalling pathway in C. elegans. Mutation of the TRPV channel subunit osm-9 and the Gα subunit gpa-3 fully abolished avoidance of (A) 25 μM SST-28 in Sstr2 transgenic animals and (B) 25 μM MIP-1α in CCR5 transgenic animals. Mutation of Gα subunit odr-3 did not affect these responses. (C) In contrast, mutation of either gpa-3 or odr-3 did not have a significant effect on avoidance response in sra6::CCR5 transgenic animals. Each data point represents an average of at least 3 independent assays for wild type and each respective strain. Error bars denote the standard error of the mean. Asterisks indicate a statistically significant difference between osm-9 or gpa-3 mutant animals and wild type animals carrying the Sstr2 or CCR5 transgenes (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005).
Teng et al. BMC Biology 2006 4:22 doi:10.1186/1741-7007-4-22