Research article
Wnt5 signaling in vertebrate pancreas development
-
* Corresponding author: Stephen C Ekker ekker001@mail.med.umn.edu
1 Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455 USA
2 Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095 USA
3 Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455 USA
4 Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455 USA
BMC Biology 2005, 3:23 doi:10.1186/1741-7007-3-23
Published: 24 October 2005Additional files
(QuickTime format) WT movie. Figure 1C–H panels were taken from this movie at the indicated time points. GFP-positive cells are first observed around the 14 somite stage as bilateral patches in insulin:GFP transgenic embryos. These cells divide and actively migrate to the posterior. The net movement towards the midline is an indirect result of convergent-extension since active migration towards the midline is not observed. By the 24 hpf stage, GFP-positive cells are coalesced into a single islet in the posterior and midline relative to the starting position.
Format: MOV Size: 1.7MB Download file
Playing the movie within this page requires QuickTime and JavaScript. Read more
Additional File 2:
Two color time-lapse imaging shows that GFP-positive cells migrate relative to the neighboring cells. (A) GFP-positive cells are first visible posterior to rhodamine-labeled cells as bilateral lows of cells. (B-G) GFP-positive cells migrate posteriorly and medially, whereas rhodamine-labeled cells do not change their relative position. (H) At 24 hpf, all GFP-positive cells coalesced into a single islet, but the rhodamine-labeled cells remain separated at their original position. Arrows: rhodamine-labeled cells; t: time (minutes).
Format: JPEG Size: 2.5MB Download file
(QuickTime format) FZ-2 morphant movie. Figure 1I–N panels were taken from this movie at the indicated time points. As also noted in non-injected insulin:GFP transgenic embryos, GFP-positive cells are first observed around the 14 somite stage as bilateral patches, and these cells divide normally in fz-2 MO-injected embryos. However, the uniform migration towards the posterior observed in the non-injected transgenic embryo is not observed in fz-2 MO-injected embryos. Instead, the polarity of cell migration is entirely lost, with no net movement along the anterior-posterior axis observed for these cells (see text).
Format: MOV Size: 1.4MB Download file
Playing the movie within this page requires QuickTime and JavaScript. Read more
Additional File 4:
Genotyping of embryos with normal and abnormal insulin expression. (A) 90% of embryos with abnormal insulin expression are carriers of ppthi1780b. Lane 4 and lane 15 represent wild-type embryos with abnormal insulin expression. (B) Control PCR for exon 1 of wnt-5, N = water only control. (C) None of the embryos with normal insulin expression are carriers of ppthi1780b. (D) Control PCR for exon 1 of wnt-5, N = water only control.
Format: JPEG Size: KB Download file
