Figure 5.

hLsg1 localized to the nucleus upon Leptomycin B treatment. (A) Subcellular localization of YFP tagged hLsg1 mutants – HeLa cells were transiently transfected with YFP-hLsg1-1-600 (1) or YFP-hLsg1-480-658 (2), or co-transfected with YFP-hLsg1-480-658 and SRP19-MRFP (3), and visualized by fluorescence microscopy. (B) Localization of hLsg1 and mutants upon Leptomycin B treatment. HeLa cells were transiently transfected with hLsg1-YFP (a), YFP-hLsg1-1-600 (b), or YFP-hLsg1-480-658 (c), treated with 15 nM Leptomycin B, fixed at 0 h, 3 h and 5 h, and visualized by fluorescence microscopy.