BMC Biology

official impact factor 5.20

Open Access Research article

How many novel eukaryotic 'kingdoms'? Pitfalls and limitations of environmental DNA surveys

Cédric Berney*, José Fahrni and Jan Pawlowski

Author Affiliations

Department of Zoology and Animal Biology, University of Geneva, CH – 1211 Geneva 4, Switzerland

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BMC Biology 2004, 2:13 doi:10.1186/1741-7007-2-13

Published: 4 June 2004

Additional files

Additional File 1:

Supplementary Figure 1. Illustration of the methods we used for the detection of chimeric sequences.

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Additional File 2:

Supplementary Table 1. Identification of the 81 environmental eukaryotic sequences we obtained from our samples of the small river, the Seymaz (Geneva, Switzerland). Two phylotypes of undetermined taxonomic position are highlighted in blue.

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Additional File 3:

Supplementary Table 2. Identification of the 403 published, environmental eukaryotic sequences we re-analysed. Previously undetected chimeras are highlighted in pink. Phylotypes previously considered as novel eukaryotic lineages, which are in fact fast-evolving members of known groups are highlighted in orange. Phylotypes that could be identified thanks to an increasing taxon sampling are highlighted in green. Remaining phylotypes of undetermined taxonomic position are highlighted in blue.

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