Open Access Research article

Host insulin stimulates Echinococcus multilocularis insulin signalling pathways and larval development

Sarah Hemer1, Christian Konrad1, Markus Spiliotis1, Uriel Koziol1, Dominik Schaack2, Sabine Förster1, Verena Gelmedin1, Britta Stadelmann3, Thomas Dandekar2, Andrew Hemphill3 and Klaus Brehm1*

Author Affiliations

1 University of Würzburg, Institute of Hygiene and Microbiology, Josef-Schneider-Strasse 2, D-97080 Würzburg, Germany

2 Department of Bioinformatics, University of Würzburg, Biocenter am Hubland, D-97074 Würzburg, Germany

3 Institute of Parasitology, Vetsuisse Faculty, University of Berne, Länggass-Strasse 122, CH-3012 Bern, Switzerland

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BMC Biology 2014, 12:5  doi:10.1186/1741-7007-12-5

Published: 27 January 2014

Additional files

Additional file 1:

The Echinococcus multilocularis life cycle. Schematic representation of the E. multilocularis life cycle and suggested actions of host insulin on parasite development/physiology.

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Additional file 2:

Amino acid sequence comparisons between Echinococcus and human insulin receptors. Pileups of LBDs and TKDs of insulin receptors from Echinococcus and human origin.

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Additional file 3:

Gene expression profiles of Echinococcus insulin signalling components during the life cycle. Diagram displaying transcriptome data concerning the expression of emir1, emir2, emilp1, and emilp2 in larval and adult stages.

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Additional file 4:

Testing the anti-EmIR1 and anti-EmIR2 antisera. File showing Western blot and immunoprecipitation analyses concerning EmIR1 and EmIR2 using the generated antisera.

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Additional file 5:

In silico analyses. Figure showing in silico models for the binding of HNMPA(AM)3 to the TKD of EmIR1.

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Additional file 6:

Components of the E. multilocularis insulin signalling pathways. Table with E. multilocularis genes predicted to be involved in insulin signalling pathways according to the E. multilocularis genome sequencing project.

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Additional file 7:

Amino acid sequence comparison of PKB components from different organisms. File showing sequence alignment of the E. multilocularis PKB-homolog EmAkt with PKBs from human and Drosophila origin.

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Additional file 8:

Amino acid sequence comparison of 4E-BP orthologs from different origin. File showing sequence alignment of the E. multilocularis 4E-BP ortholog with those of human and insect origin.

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