Table 7

FFAT-like proteins identified in VAPA and VAPB immunoprecipitates by mass spectrometry
Protein name UniProt IDa MW (Da)a FFAT motifa Share of spectrum IDsb
Flag-VAPA (U2OS) Flag-VAPB (U2OS) Endogenous VAPB
HeLa cells Mouse brain
FAF1 Q9UNN5 73954 DFEDATE 0.85% 0.68% 0.06% -
ASNA1 O43681 38793 EFEDAPD 0.69% 0.79% 0.20% -
RAB3GAP1 Q15042 110524 EFFECLS 2.65% 1.79% 0.20% 0.43%
RAB3GAP2 Q9H2M9 155985 NA 1.73% 1.61% 0.10% 0.37%
WDR44 Q5JSH3 101366 EFYDAPE 1.97% 1.66% - 0.05%
STX1Bc P61266 33245 EFFEQVE - - - 0.20%
STX1Ac Q16623 33023 EFFEQVE - - - 0.03%

aThe UniProt ID, MW and FFAT motif are indicated for the human proteins.

bThe share of spectrum IDs is indicated as a measure of protein abundance in the immunoprecipitates.

cSTX1A and B are shown in italics because their putative FFAT-like motifs could not be confirmed experimentally.

NA, not applicable.

Baron et al.

Baron et al. BMC Biology 2014 12:39   doi:10.1186/1741-7007-12-39

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