Table 4

Ubiquitinated targets of VAPB and FAF1 identified by mass spectrometry upon enrichment of ubiquitinated peptides
Protein name UniProt ID GlyGly peptidesa (times identified)b
RPN2 P04844 LSK154EETVLATVQALQTASHLSQQADLR (4×)
K244NFESLSEAFSVASAAAVLSHNR (2×)
LHNQK442TGQEVVFVAEPDNK (3×)
DDOST P39656 APTIVGK189SSLNPILFR (2×)

aA mixture of light-labeled Flag-VAPB and heavy-labeled Flag-FAF1 immunoprecipitates was analyzed by mass spectrometry after ubiquitinated peptide enrichment using antibodies specific to lysine-ϵ-GlyGly. The lysine residues carrying the GlyGly modification are indicated in bold and the superscript is the position in the protein sequence.

bThe numbers in parenthesis indicate how many times each peptide was identified by mass spectrometry.

Baron et al.

Baron et al. BMC Biology 2014 12:39   doi:10.1186/1741-7007-12-39

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