Table 1

TCR α and β chain repertoires of a Th1 polarized T cell line
CDR3 region CDR3 length %
TCR alpha EDSAIYFC AASREGTGSKLS FGKG 12 34
SDSAKYFC ALEGRGGRALI FGTG 11 20
EDSGTYFC AALPGTGSNRLT FGKG 12 18
GDSAMYFC AAKNSGTYQR FGTG 10 8
GDSAIYFC SASMTNNNNRIF FGDG 12 6
EDSGTYFC AADSNYQLI WGSG 9 4
EDSGTYFC AAETNSAGNKLT FGIG 12 2
EDSGTYFC AADSNHQLI FGSG 9 2
EDSGTYFC AAEAANYNVLY FGSG 11 2
EDSAIYFC AASKPNNRIF FGDG 10 2
SDSAVYFC ALSALGTGNYKYV FGAG 13 2
TCR beta DDSATYFC ASSQGPLSNERLF FGHG 13 22
NEMAVLFC ASSRSGDQDTQY FGPG 12 18
EDSAVYLC ASSRDWGDTQY FGPG 11 14
DDSATYFC ASSQEMQGQDTQY FGPG 13 6
EDSAVYLC ASSPWGVQDTQY FGPG 12 4
QDSAVYLC ASSLAGQGARSQNTLY FGAG 16 4
SQTSLYFC ASSPGSNERLF FGHG 11 4
KDSAVYLC ASSLVGAEQF FGPG 10 4
DDSATYFC ASSKAGTGEDTQY FGPG 13 2
DDSATYFC ASSQQGDQDTQY FGPG 12 2
DDSATYFC ASSQEGTGVQDTQY FGPG 14 2
DDSATYFC ASSQEGTGGDEQY FGPG 13 2
DDSATYFC ASSQEGLSSYEQY FGPG 13 2
DDSATYFC ASSQEGLGNYEQY FGPG 13 2
DDSATYFC ASSQEMQGDQDTQY FGPG 14 2
QDSAVYLC ASSNQNYAEQF FGPG 11 2
QDSAVYLC ASSSRDWGDEQY FGPG 12 2
EDSAVYFC ASSQAGTDTQY FGPG 11 2
EDSAVYFC ASSSPGGSYEQY FGPG 12 2
SQTSVYFC ASGDSQGANQAPL FGEG 13 2

T cell lines were maintained in culture for one round of re-stimulation under highly polarizing conditions before taking RNA for TCR sequence analysis. TCRα and β chain repertoire analysis of an H2-Ag7/PLP 56-70 specific Th1, Th2 and Th17 cell lines shows expansion of distinct, dominant TCRα chains in the absence of a dominant β chain. The frequency (%) of each unique TCR sequence identified is shown. CDR3 length is defined as the number of amino acids between the invariant C residue and the F/W-G-X-G motif. TCRα and TCRβ CDR3 region lengths are shown in each table. Data are representative of 50 TCRα and 50 TCRβ sequences for each T cell line. The mean CDR3 length for the TCRα chain is 12.2 ± 0.2 (SE) for Th2 cultures, 11.4 ± 0.1 (SE) for Th1 cultures, and 11.1 ± 0.1 (SE) for Th17 cultures.

The most dominant TCR alpha sequence is shown in bold.

Mean CDR3α length 11.42 + 0.13 (SE) (n = 50).

Mean CDR3β length 12.30 + 0.17 (SE) (n = 50).

Reynolds et al.

Reynolds et al. BMC Biology 2014 12:32   doi:10.1186/1741-7007-12-32

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