Impact of different in vivo peptide priming doses on ex-vivo cytokine program. Th2αβ TCR transgenic mice were primed in one hind footpad with 5 μg/ml (open bars), 25 μg/ml (gray bars) or 125 μg/ml (black bars) PLP 56-70 peptide in CFA. At Day 10 after immunization, DLN cells were re-stimulated with peptide at 0.1, 1.0, 10 or 100 μg/ml as indicated on the x-axis and assayed in triplicate cultures for IFNγ production by ELISA (A). RNA was prepared from primed DLN immediately ex-vivo for real-time PCR analysis of GATA-3 and RORγt transcription (B).
Reynolds et al. BMC Biology 2014 12:32 doi:10.1186/1741-7007-12-32