Figure 5.

TCRVαβ transgene pair impacts on cytokine production and transcriptional profile. (A) TCRαβ transgenics or littermate controls were primed with peptide in CFA and DLN cells harvested at Day 10. 3H-thymidine incorporation and cytokine production was determined from TCRαβ transgenics (filled triangles; n = 6) and controls (filled squares; n = 10). Error bars indicate ± SE. This is representative of three separate experiments. (B) TCRαβ transgenic cell lines (black bars) (n = 3) and littermate control lines (white bars) (n = 5) were established from primed DLN cells from mice primed 10 days earlier with PLP56 to 70/CFA and re-stimulated every 10 days through to four cycles in the absence of any exogenous polarization. (C) At each re-stimulation the relative expression of IFNγ, GATA-3 and RORγT was determined. Error bars indicate SE. (D) TCRαβ (filled triangles; n = 10), TCRα chain transgenics (filled circles; n = 12), and littermate controls (filled squares; n = 27) were primed with peptide/IFA and at Day 10, DLN cells were harvested and 3H-thymidine incorporation and cytokine production analyzed. These data are representative of three independently performed experiments. CFA, Complete Freund’s adjuvant; DLN, draining lymph nodes.

Reynolds et al. BMC Biology 2014 12:32   doi:10.1186/1741-7007-12-32
Download authors' original image