Schematic diagram illustrating construction of the Th2 derived TCRα chain transgene with an elongated CDR3 region, Th1 TCRα chain transgene with a short CDR3 region, and Th2 TCRβ chain transgene. (A) The Th2-derived TCRα chain transgene with an elongated CDR3 region, (B) Th1 TCRα chain transgene with a short CDR3 region, and (C) Th2 TCRβ chain transgene showing the recombined V and J gene segments and the amino acid sequence of the CDR3 regions. Intronic sequences flank the 3′ and 5′ ends of the coding regions and also separate the leader sequence (LP1) from the main coding region of the V gene. Transgenes were cloned into TCR α or β chain expression vectors that contain TCRα or β constant regions and endogenous promoter elements, using Xma I/Sac II sites for the TCRα and Xho I/Sac II sites for the TCR β transgene.
Reynolds et al. BMC Biology 2014 12:32 doi:10.1186/1741-7007-12-32