Pluripotency inducing factors sox2, c-myc and klf4, and eye field transcriptional factor expression is increased in the retinal pigmented epithelium after retina removal. Quantitative RT-PCR analysis at 6, 24 and 72 hours post-retinectomy (injury) of (A)sox2, c-myc and klf4; (B) eye field transcriptional factors six3, six6, lhx2 and rx1 and the progenitor markers ascl1 and chx10; and (C) Retinal pigmented epithelium (RPE)-specific factors mitf and tyr. The expression levels were normalized with intact RPE (no injury). The analysis was performed using three independent biological samples (n = 3) in triplicate and the comparative cycle threshold (2-ΔΔCt) method was used to determine relative changes in transcripts compared with gapdh mRNA levels. Significance was determined with unpaired Student’s t-test by comparing each time point with the intact RPE (no injury). Error bars represent standard error of the mean. *P < 0.05; **P < 0.01; ***P < 0.001, compared with intact RPE. NS, non-significant; RPE, retinal pigmented epithelium.
Luz-Madrigal et al. BMC Biology 2014 12:28 doi:10.1186/1741-7007-12-28