Additional file 4: Figure S4.

EdU is incorporated into proliferating archeocytes in the inner cell mass in A. queenlandica larvae. A, B: Confocal sections of the inner cell mass in a free-swimming larva pulse-labelled with EdU. Nuclei are labelled with DAPI. Arrowheads show EdU-positive nuclei. The boxed region in B is magnified in an inset. Note that the EdU-positive nuclei are 3.5 to 4.0 μm in diameter and contain prominent nucleoli (nu), as observed in larval archeocytes in the inner cell mass in another demosponge Haliclona tubifera[44]. C: A transmission electron micrograph showing prominent nucleoli (nu) in nuclei of archeocytes (ar) in the inner cell mass within an A. queenslandica larva. Scale bar: 10 μm (A, B), 5 μm (C).

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Nakanishi et al. BMC Biology 2014 12:26   doi:10.1186/1741-7007-12-26