Additional file 3: Figure S3.

Larval ciliated epithelial cells lose cilia and transdifferentiate into choanocytes via an archeocyte intermediate during metamorphosis in A. queenslandica. Confocal sections through a flask cell (fc) at the free-swimming larva stage (A) and a transdifferentiating flask cell at the settlement stage labelled with CM-DiI (B). Note the loss of apical structures including the cilium (ci). C: Confocal sections viewed from the top at settlement, showing that many DiI-labelled cells are internalised (arrowheads). D: Confocal sections viewed from the top during metamorphosis; note that DiI-labelled cells have differentiated into archeocytes with characteristic large nucleoli (nu; inset magnified from the boxed cells). E: Confocal sections of individual archeocytes of the larval epidermal origin undergoing further differentiation at the chamber-formation stage; an arrowhead shows a differentiating choanocyte with a cilium (ci in inset) whose cell body has yet to complete cytokinesis within the mother archeocyte (ar). F: Confocal sections of a multi-nucleated archeocyte-choanocyte intermediate at the chamber-formation stage. Differentiating choanocytes with cilia (ci) are evident (arrowheads). Abbreviations: ap apical non-ciliary process; ba basal process; co columnar epithelial cell. Scale bar: 10 μm (A, B, inset in D, E, F), 100 μm (C, D).

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Nakanishi et al. BMC Biology 2014 12:26   doi:10.1186/1741-7007-12-26