Figure 7.

Aberrant gene expression in hindstomach in Isl1MCM/Del mutants. (A) RT-qPCR analysis of mRNA levels of hindstomach-enriched transcription factors at E14.5 indicates significant reduction of α-SMA, Six2 Nkx2.5, Gata3, and Gremlin mRNA in Isl1MCM/Del mutant stomachs (n = 4). All results were normalized to levels of Gapdh mRNA. (B) RT-qPCR analysis of mRNA levels of hindstomach-enriched transcription factors at E18.5 indicates a significant reduction of Nkx2.5, Gata3, and Gremlin mRNA in the Isl1MCM/Del mutant stomachs (n = 4). All results were normalized to levels of Gapdh mRNA. Data are mean ± SEM (n = 6 mice per group). *P <0.05 versus Isl1F/+; **P <0.01 versus Isl1F/+ (Student’s t-test). (C-F) WISH mRNA analysis confirmed loss of Isl1, Gata3, Gremlin, and Nkx2.5 mRNA expression at E14.5 in the Isl1MCM/Del mutant stomachs. Isl1 and Gata3 mRNA were severely down-regulated in Isl1MCM/Del mice, whereas Gremlin and Nkx2.5 expression were slightly reduced. Arrows point to the pyloric sphincter.

Li et al. BMC Biology 2014 12:25   doi:10.1186/1741-7007-12-25
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