Additional file 4: Figure S1.

Morphology of ENCCs and filopodia and lamellopodia extension. Figure S2: Interactions between the filopodia and/or lamellipodia at the front of one chain and cells or filopodia from another chain resulted in three general outcomes: adhesive, “walk-past” and repulsive (contact inhibition of locomotion). Figure S3: There were no significant differences in the number of Hu + neurons per area of Kik + cells in control explants (n = 12) and in explants cultured in the presence of BQ788 (20 μM) (n = 13) for 10 hours. At the end of the culture period, explants were fixed, processed for immunohistochemistry using an antibody to the pan-neuronal marker, Hu. The number of Hu + cells per area of Kik + cells was quantified using Fiji software.

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Young et al. BMC Biology 2014 12:23   doi:10.1186/1741-7007-12-23