Analysis of growth and cell cycle progression following RNAi of Tb11.NT.28. (A)Tb11.NT.28 RNAi in procyclics (PC). Growth for uninduced (-tet) and induced (+tet) cells shown in log scale. (B) Analysis of the K/N configurations after induction of Tb11.NT.28 RNAi. Cells were scored every 24 hours as having normal DNA (one kinetoplast and one nucleus (1K1N), two kinetoplasts and one nucleus (2K1N) or two kinetoplasts and two nuclei (2K2N)) or abnormal DNA content (one kinetoplast and no nucleus (1K0N), one kinetoplast and two nuclei (1K2N) and more than two nuclei (>2 N). Cell counts were done as described in the legend for Figure 5.
Ericson et al. BMC Biology 2014 12:14 doi:10.1186/1741-7007-12-14