Localization of the seven small proteins essential in the procyclic life cycle stage. Anti-HA or anti-GFP antibodies were used to detect C-terminal HA- (Tb11.NT.28 and Tb11.NT.29) or GFP- (Tb10.NT.87, Tb10.NT.90, Tb10.NT.86, Tb3.NT.18, and Tb11.NT.108) tagged versions of the proteins by fluorescence microscopy. DNA was stained with Hoechst (blue) and DIC images are shown in the right panels. DIC, differential interference contrast.
Ericson et al. BMC Biology 2014 12:14 doi:10.1186/1741-7007-12-14