Additional file 5: Figure S5.

Analysis of Notch vesicle co-localization with Avl and Hrs. In awd mutant cells, Notch accumulates in Avl-positive and Hrs-negative early endosomes. Stage 7–8 egg chambers were dissected from hs-flp/GbeSu(H)m8-lacZ; act-Gal4, UAS-GFP/+; FRT82B, act-Gal80/FRT82B, awdj2A4 females and stained for NICD and Avl (A,A’) or NICD and Hrs (B,B’). Co-localization was analyzed by using ImageJ. The Pearson's coefficient ranges from +1 = complete correlation to −1 = anti-correlation, with 0 = no correlation. The mean values (n = 4) of Pearson’s coefficients for NICD and Avl (A) and for NICD and Hrs (B) in awd+ and awd mutant cells were plotted together with standard deviations (error bars). Statistical significance was calculated using the two-tailed paired t-test (** = P <0.01; N.S. = No Significant). (A’) Co-localization image of NICD and Avl based on the analysis of awd mutant cells and neighboring awd+ cells showed in Figure  6A. (B’) Co-localization image of NICD and Hrs based on the analysis of awd mutant cells and neighboring awd+ cells.

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Ignesti et al. BMC Biology 2014 12:12   doi:10.1186/1741-7007-12-12