Notch signaling defect in awd mutant cells is rescued by exogenous NICD. Stage 7–8 egg chambers were dissected from females of the genotype hs-flp/GbeSu(H)m8-lacZ; act-Gal4, UAS-GFP/UAS-NICD; FRT82B, act-Gal80/FRT82B, awdj2A4 (A-B) or hs-flp/GbeSu(H)m8-lacZ; act-Gal4, UAS-GFP/UAS-NEXT; FRT82B, act-Gal80/FRT82B, awdj2A4 (C-E). (A, C) As controls, NICD and NEXT expression is verified. (B) A stage 7 egg chamber with MARCM clones of awd (Awd-negative and GFP+, marked by lines) simultaneously expressing NICD. The expression of endogenous Hnt (red) is restored in a majority of the mutant cells. The Awd staining is in cyan. Note that the diffused Awd staining in regions abutting the apical side of the follicle cells is within the germline cells, occasionally observed in abnormal egg chambers. (D-E) Exogenously expressed NEXT cannot rescue the awd mutant phenotype. awd MARCM mutant clones lacking Awd staining (cyan) marked by the GFP expression and indicated by lines show loss of GbeSu(H)m8-lacZ reporter gene expression (red in D) as well as loss of Hindsight (Hnt) expression (red in E). Bars in (A-B) are 20 μm. Bars in (C-E) are 10 μm. NEXT, Notch external truncation; NICD, Notch intracellular domain.
Ignesti et al. BMC Biology 2014 12:12 doi:10.1186/1741-7007-12-12