Additional file 7: Movie 5.


Live cell imaging of an uninfected MDM expressing PH-GFP. MDMs were nucleofected to express PH-GFP and imaged after 24 hours using an UltraVIEW Vox spinning disc confocal system fitted on a Nikon ECLIPSE Ti microscope equipped with a temperature and CO2-controllable environment chamber. The movie was assembled from images taken every 10 seconds. Cell as shown in Figure 3B.

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Mlcochova et al. BMC Biology 2013 11:89   doi:10.1186/1741-7007-11-89