Additional file 5: Figure S2.
Immunostaining for PI(4,5)P2 in MDMs. MDMs were either (A) fixed with 4% paraformaldehyde/2% glutaraldehyde and permeabilized with 0.5% saponin or (B) fixed with 4% paraformaldehyde and permeabilized in 0.2% Triton X-100. Cells were labeled with a mouse monoclonal anti-PI(4,5)P2 antibody 2C11 and co-stained for CD81. Scale bars: 10 μm.
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Mlcochova et al. BMC Biology 2013 11:89 doi:10.1186/1741-7007-11-89