Additional file 11: Movie 6.


3D reconstruction of an infected MDM labeled with CellMask and treated with latrunculin A. MDMs infected for 7 days with HIV-1BaL were treated with DMSO (control) or latrunculin A for 2 hours and labeled with CellMask. Fiji software was used to build a 3D reconstruction of an IPMC from 200 optical z-slices (step size of 0.04 μm). The 3D reconstructions were cut out from whole cells to display the individual compartments. The control cell is shown on the left, and the reconstruction from a latrunculin-treated cell on the right. Note multiple connections to the cell surface.

Format: MOV Size: 7MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Mlcochova et al. BMC Biology 2013 11:89   doi:10.1186/1741-7007-11-89