Additional file 10: Figure S5.
HIV particles still assemble in IPMCs after treatment with latrunculin A. HIV-infected MDMs were treated with DMSO or 2 μM Latrunculin A for 2 hours and processed for cryosectioning. Ultrathin cryosections from (A, B) infected control or (C, D, E) latrunculin A-treated macrophages were immunolabeled with anti-p24 antibodies, a rabbit anti-mouse bridging antibody and protein A-gold (5 nm in A, B and D, or 10 nm in C and E). EM analysis showed that HIV particles accumulated in complex IPMCs in both control and latrunculin A-treated cells. Arrows in B and D show representative immature virus particles and budding profiles, indicating virus assembly within IPMCs. Arrowheads in E mark an example of the electron-dense coats containing β2-integrins and focal adhesion proteins . Images were taken on an FEI Tecnai G2 Spirit transmission EM with a Morada 11 MegaPixel TEM camera and AnalySIS software. Scale bars: 1 μm in A and C, and 200 nm in B, D, E.
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Mlcochova et al. BMC Biology 2013 11:89 doi:10.1186/1741-7007-11-89