Egg production in response to corpora allata knockout and rescue by JHA. A) Daily per capita fecundity of mated females in cages. Control genotypes with intact corpora allata: Aug21, GFP and UAS-NIPP1. Corpora allata knockout (CAKO): Aug21,GFP; UAS-NIPP1. B) Daily per capita fecundity of mated females in cages: wildtype (w1118) and CAKO mated females exposed to vehicle control (EtOH) or to methoprene (JHA). In (A) and (B) error bars are standard deviation. C) Total eggs per mated, individually held females (age 2 to 10 days). Whisker plots show individual values, group means (wildtype, 764 eggs; CAKO, 365 eggs), one standard deviation (wildtype, 74 eggs; CAKO, 140.6 eggs) and range. D) Distribution of ovariole maturation scored by oocyte stage from mated females aged 10 days; wildtype and CAKO females, exposed to vehicle or JHA. The percentage is among ovarioles of one ovary, averaged among 842 (wildtype no JHA), 71 (wildtype with JHA), 849 (CAKO no JHA) and 780 (CAKO with JHA) females per group. Oocyte stage categories: <6, the most mature egg chamber in ovariole has no yolk; 5/6, the most mature egg chamber in ovariole has little yolk; Discont. (discontinuous), ovariole contains one mature (or hypertrophied mature) egg chamber but next most mature egg chamber contains no yolk (stage <6); Matureovariole has a continuous sequence of mature and maturing egg chambers with at least one chamber between stage 7 and 12.
Yamamoto et al. BMC Biology 2013 11:85 doi:10.1186/1741-7007-11-85