Table 1

Relative abcb4 and abcb5 mRNA abundances in zebrafish embryos at different developmental stages
hpf 1 6 12 24 48
abcb4 57.09 ± 13.03 2.36 ± 0.91 0.91 ± 0.26 2.03 ± 0.31 11.62 ± 0.99
abcb5 11.81 ± 1.87 2.05 ± 0.74 7.81 ± 0.74 2.36 ± 1.19 0.614 ± 0.09
β-actin 20,575 ± 572 15,613 ± 6,938 24,639 ± 9,007 17,740 ± 3,948 22,004 ± 6,891

The mRNA abundances were determined in embryos at 1, 6, 12, 24 and 48 hours post fertilization (hpf). The expression levels that were normalized to 18S rRNA are shown as means ± SD of four independent RNA isolations. Expression values were multiplied by 106 for clarity of presentation. Beta-actin transcript levels are exemplarily shown for a highly expressed gene for comparison.

Fischer et al.

Fischer et al. BMC Biology 2013 11:69   doi:10.1186/1741-7007-11-69

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