Bacteria tracking by in vivo magnetic resonance imaging
- Equal contributors
1 Department of Clinical Radiology, University Hospital Münster, Münster 48149, Germany
2 Institute of Medical Microbiology, University Hospital Münster, Münster 48149, Germany
3 Fluorescence Microscopy Facility Münster, Institute of Medical Physics and Biophysics, University Hospital Münster, Münster 48149, Germany
4 Department of Dermatology, University Hospital Münster, Münster 48149, Germany
5 Institute of Medical Physics and Biophysics, University Hospital Münster, Münster 48149, Germany
6 Institute of Inorganic and Analytical Chemistry, University of Münster, Münster 48149, Germany
BMC Biology 2013, 11:63 doi:10.1186/1741-7007-11-63Published: 28 May 2013
Additional file 1:
Fluorescence microscopy of labeled S. aureus bacteria.S. aureus labeled with Syto 9 (green) and rhodamine-coated iron oxide particles (red). (A) screenMAG 100 nm polysaccharide, (B) screenMAG 100 nm diethylamine ethyl, (C) screenMAG 100 nm phosphatidyl-cholin, (D) screenMAG 100 nm poly(maleic acid-co-olefin).
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Additional file 2:
Fluorescence microscopy of labeled E. coli bacteria.E. coli labeled with Syto 9 (green) and rhodamine-coated iron oxide particles (red) by using either (A) amine-coated 5-nm IONPs or (B) citrate-coated 5-nm IONPs.
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Murine macrophages phagocytizing iron-labeled bacteria. The movie shows how iron-labeled S. aureus bacteria (red) are phagocytized by murine macrophages.
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Additional file 4:
Longitudinal and transverse relaxation times of iron-labeled S. aureus cultures, measured at different growth stages. The measurements of the relaxivities showed that T1 did not change significantly over the time course, while T2 was significantly decreased at the beginning of the growth curve and increased steadily until a stationary value was reached after 3 h.
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Additional file 5:
MR images of the left footpad injected with IONPs. (A) IONPs in the footpad were observed as hypointensities (yellow arrow) in axial T2*-weighted MRI. (B) T2-weighted images of the popliteal lymph node on Day 2 post injection did not show hypointensities as was observed for a footpad infection induced by iron-labeled bacteria.
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